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We are now shipping live cells, plasmids, genomic DNA, and media packets, although shipping may take somewhat longer than normal. Contact us with any questions.


Stock IDSD02458
Strain NameHTA3-T42F1
AKA
SpeciesT. thermophila
Genetic Descriptionhtaz[∆::neo2]/htaz[∆::neo2]; mpr1-1/MPR1-1? (htaz[∆::neo2]/htaz[K130R]; pm-r)
MAC Knockins/outsHTAZ coding replaced with mutation K130R
MIC Knockins/outsNeo2 in HTAZ
Gene of InterestHTA3 (TTHERM_00143660)
Origin
PublicationsRen Q and Gorovsky MA. 2003. The nonessential H2A N-terminal tail can function as an essential charge patch on the H2A.Z variant N-terminal tail. Mol Cell Biol 23(8):2778-89.

Ren Q and Gorovsky MA. 2001. Histone H2A.Z acetylation modulates an essential charge patch. Mol Cell 7(6):1329-35.
CommentsG304A1xG311A1 ∆HTA3 knockout heterokaryon. Rescued with HTAZ mutated K130R. no ubiquitination of H2AF/Z. Can mate with T42E1

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD02458
Requires MTA?No
Academic cost $45