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We are now shipping live cells, plasmids, genomic DNA, and media packets, although shipping may take somewhat longer than normal. Contact us with any questions.

Stock IDSD02403
Strain Namehta1-K123R+htz1 K130R-G2b
SpeciesT. thermophila
Genetic Descriptionhtax[∆::neo2]/htax[∆::neo2]; hta1[∆::neo2]/hta1[∆::neo2]; htaz[∆::neo2]/htaz[∆::neo2] (htax[K123R]/htax[∆::neo2]; hta1[∆::neo2]/hta1[∆::neo2]; htaz[K130R]/htaz[∆;;neo2]; pm-r, ?)
MAC Knockins/outsmutant HTAX and HTAZ rescue of mac KO
MIC Knockins/outsNeo2 knockout of HTAX, HTA1, HTAZ
Gene of InterestHTA1 (TTHERM_00790790), HTA2 (TTHERM_00316500), HTA3 (TTHERM_00143660)
OriginZhe Wang
PublicationsWang Z. 2007. Functional studies of histone ubiquitylation in tetrahymena thermophila. University of Rochester.

Wang Z, Cui B, Gorovsky MA. 2009. Histone H2B ubiquitylation is not required for histone H3 methylation at lysine 4 in tetrahymena. J Biol Chem 284(50):34870-9.
CommentsHTAX/HTAZ double mutant rescue; Rescue of h∆∆HTA∆HTZ1-F1B1 and B1C5 with Dryl's.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD02403
Requires MTA?No
Academic cost $45