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We are now shipping live cells, plasmids, genomic DNA, and media packets, although shipping may take somewhat longer than normal. Contact us with any questions.

Stock IDSD02376
Strain NameHHT2S10AS28A-4
SpeciesT. thermophila
Genetic Descriptionhht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆,::neo2]/hhf2,hht2[∆,::neo2] (hht1[∆::neo2]; hhf2,hht2[∆,::neo2]/ hht2[S10A,S28A]; pm-r, ?)
MAC Knockins/outsKO of HHT1, HHF1;and for HHT2 have mutations S10A and S28A
MIC Knockins/outsNeo knockout of HHT1,HHT2, HHF1
Gene of InterestHHT2 (TTHERM_00189180)
OriginJody Bowen
PublicationsWei Y, Yu L, Bowen J, Gorovsky MA, Allis CD. 1999. Phosphorylation of histone H3 is required for proper chromosome condensation and segregation. Cell 97(1):99-109.

Cui B, Liu Y, Gorovsky MA. 2006. Deposition and function of histone H3 variants in tetrahymena thermophila. Mol Cell Biol 26(20):7719-30.
CommentsMate Yifan Liu’s ∆H3 2-6 x ∆H3 10-6 homozygous heterokaryons (∆HHT1 with neo, and ∆HHT2/HHF2 with neo2 in mic) assorted to Paromomycin sensitive in mac. Shoot with pHHT2S10AS28A #6-later after seq.transformant found a wrong nucleotide~25 bases upsteam of ATG, transformant seems normal.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD02376
Requires MTA?No
Academic cost $45