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We are now shipping live cells, plasmids, genomic DNA, and media packets, although shipping may take somewhat longer than normal. Contact us with any questions.

Stock IDSD02307
Strain NameGTU1/CU522 T146GHA #8
SpeciesT. thermophila
Genetic Descriptionbtu1-1[K350M]/btu1-1[K350M (btu1-1[K350M]/btu1-?[MTT1p,,∆,(::GTU1,T146G),HAc]; tax-r, ?)
MAC Knockins/outsGTU1 with mutation T146G and c-terminal HA tag into the BTU1 locus under control of MTT1 promoter. Not sure if complete replacement.
MIC Knockins/outs
Gene of InterestGTU1 (TTHERM_00079520)
OriginYuhua Shang
PublicationsShang Y, Li B, Gorovsky MA. 2002. Tetrahymena thermophila contains a conventional gamma-tubulin that is differentially required for the maintenance of different microtubule-organizing centers. J Cell Biol 158(7):1195-206.

Shang Y, Tsao CC, Gorovsky MA. 2005. Mutational analyses reveal a novel function of the nucleotide-binding domain of gamma-tubulin in the regulation of basal body biogenesis. J Cell Biol 171(6):1035-44.
CommentspT146G HA somatic transformant in CU522, partial ? replacement of btu1(K350M) gene,GTU1 (T146G) with HA tag under control of MTT1 promoter. #1 and #5 are two other separate single cell lines.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD02307
Requires MTA?No
Academic cost $45