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Stock IDSD02306
Strain NameGTU1/CU522 BTGBHA #3
AKA
SpeciesT. thermophila
Genetic Descriptionbtu1-1[K350M]/btu1-1[K350M] (btu1-1[K350M]/btu1-?[MTT1p,,∆,(::GTU1),HAc]; tax-r, ?)
MAC Knockins/outsGTU1 with c-terminal HA tag into the BTU1 locus under control of MTT1 promoter. Not sure if complete replacement.
MIC Knockins/outs
Gene of InterestGTU1 (TTHERM_00079520)
OriginYuhua Shang
PublicationsShang Y, Li B, Gorovsky MA. 2002. Tetrahymena thermophila contains a conventional gamma-tubulin that is differentially required for the maintenance of different microtubule-organizing centers. J Cell Biol 158(7):1195-206.

Shang Y, Tsao CC, Gorovsky MA. 2005. Mutational analyses reveal a novel function of the nucleotide-binding domain of gamma-tubulin in the regulation of basal body biogenesis. J Cell Biol 171(6):1035-44.
CommentspBTGBHA somatic transformant in CU522, partial ? replacement of btu1(K350M) gene,GTU1 with HA tag under control of MTT1 promoter.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD02306
Requires MTA?No
Academic cost $35