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We are now shipping live cells, plasmids, genomic DNA, and media packets, although shipping may take somewhat longer than normal. Contact us with any questions.

Stock IDSD02304
Strain Namegl∆H3all, M6
SpeciesT. thermophila
Genetic Descriptionhht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆::neo2]/hhf2,hht2[∆::neo2]; hht3[∆::neo2]/hht3[∆::neo2]; mpr1-1/mpr1-1 (mp-r, VI)
MAC Knockins/outs
MIC Knockins/outsNeo3 KO of HHT!,HHT2/HHF2,HHT3
Gene of InterestHHT + HHF (TTHERM_00570560, TTHERM_00189180, TTHERM_00189170)
OriginYifan Liu
PublicationsLiu Y, Song X, Gorovsky MA, Karrer KM. 2005. Elimination of foreign DNA during somatic differentiation in tetrahymena thermophila shows position effect and is dosage dependent. Eukaryot Cell 4(2):421-31.

Liu Y. 2003. Genetic studies of RNA-mediated DNA elimination in the developing macronuclei of tetrahymena thermophila. University of Rochester.
CommentsGermline heteokaryon for all three H3 genes(major and minor) and H4II, neo cassette.It is mating type VI Note: now know there is a HHT4 which was still present

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD02304
Requires MTA?No
Academic cost $45