Skip to main content

Follow @TetrahymenaSC

We are now shipping live cells, plasmids, genomic DNA, and media packets, although shipping may take somewhat longer than normal. Contact us with any questions.

Stock IDSD02292
Strain NameDE-HA #2
SpeciesT. thermophila
Genetic Descriptiongtu1[∆::neo2]/ gtu1[∆::neo2] (gtu1[∆::neo2]; MTT1/mtt1[∆, (::GTU1, D124A,E126A, HAc)]; pm-r, ?)
MAC Knockins/outsGTU1 with mutations D124-A and E126-A and HA tag at c-teminus into the MTT1 locus, partial replacement.
MIC Knockins/outsNeo2 into GTU1 locus
Gene of InterestGTU1 (TTHERM_00079520)
OriginYuhua Shang
PublicationsShang Y, Tsao CC, Gorovsky MA. 2005. Mutational analyses reveal a novel function of the nucleotide-binding domain of gamma-tubulin in the regulation of basal body biogenesis. J Cell Biol 171(6):1035-44.

Shang Y, Li B, Gorovsky MA. 2002. Tetrahymena thermophila contains a conventional gamma-tubulin that is differentially required for the maintenance of different microtubule-organizing centers. J Cell Biol 158(7):1195-206.
CommentsSame as A2 DE-HA but these are new transformants, only 1 week old. Not single celled and genotype not tested.

pDE124/6 HA rescued GTUKO 5x6 progeny having mutation @ D124-A and E126-A in GTU1-1 gene, also one HA tag@c-terminus; driven by MTT1 promoter. Needs Cd to induce.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD02292
Requires MTA?No
Academic cost $45