Tetrahymena Stock Center| Stock ID | SD02269 |
| Strain Name | BTU1-94c (G3-B) |
| AKA | |
| Species | T. thermophila |
| Genetic Description | mic still from VB1 cell (btu1[ā2,3,4]/btu1[ā2,3,4]; BTU1 has M--- ( REI removed at start of coding); no longer taxol hypersensitive or oryzalin resistant since back to wt L350) |
| MAC Knockins/outs | Removed the amino acids REI at positions 2,3,4 ibeta tubulin 1 gene |
| MIC Knockins/outs | |
| Gene of Interest | BTU1 (TTHERM_00348510) |
| Origin | Jody Bowen |
| Publications | Gaertig J, Thatcher TH, Gu L, Gorovsky MA. 1994. Electroporation-mediated replacement of a positively and negatively selectable beta-tubulin gene in tetrahymena thermophila. Proc Natl Acad Sci U S A 91(10):4549-53. |
| Comments | Somatically transformed VB1 cells with BTU1 p94c construct. p94c changes MREI at begining of coding to remove the REI. Also put the missing āCā back into the BTU1 coding region (~ position + 1040) From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed. |
| Research Resource Identifier | TSC_SD02269 |
| Requires MTA? | No |
| Academic cost | $45 |
