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We are now shipping live cells, plasmids, genomic DNA, and media packets, although shipping may take somewhat longer than normal. Contact us with any questions.

Stock IDSD02235
Strain NameB1-1A7
SpeciesT. thermophila
Genetic DescriptionngoA[∆::neo3]/ngoA[∆::neo3] (NgoA/ngoA[∆::neo3]; pm-r, ?)
MAC Knockins/outspartial KO of NgoA with neo3
MIC Knockins/outsneo3 into coding of NgoA gene
Gene of InterestNgoA (TTHERM_00655890)
OriginXiaoyuan Song
PublicationsDou Y, Song X, Liu Y, Gorovsky MA. 2005. The H1 phosphorylation state regulates expression of CDC2 and other genes in response to starvation in tetrahymena thermophila. Mol Cell Biol 25(10):3914-22.
CommentsHeterokaryon clone of NgoA gene, germline knockout. The KO construct is neo gene driven by the MTT1 promoter, and this is in the opposite direction in NgoA region.

Mac not completely assorted, mix of KO and KO with neo3 cassette. Note some vials are completely assorted to pm-s.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD02235
Requires MTA?No
Academic cost $45