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We are now shipping live cells, plasmids, genomic DNA, and media packets, although shipping may take somewhat longer than normal. Contact us with any questions.


Stock IDSD02234
Strain NameB-E1-V5-PP36
AKA
SpeciesT. thermophila
Genetic Description(pm-r, II)
MAC Knockins/outsV5 tagged PP36 protein
MIC Knockins/outs
Gene of InterestTTHERM_00238830
OriginJody Bowen
PublicationsFor PP1 studies see:

Song X, Bowen J, Miao W, Liu Y, Gorovsky MA. 2012. The nonhistone, N-terminal tail of an essential, chimeric H2A variant regulates mitotic H3-S10 dephosphorylation. Genes Dev 26(6):615-29.
Commentssomatic mac transformation of B2086 with construct of PP36 (Protein phosphatase 36 kD MW, predicted TTHERM_00238830) V5 tag after ATG, with neo3 in reverse orientation -200 bases upstream of ATG. Localizes to nucleoli, not studied further.

Incomplete replacement, pushed to 3mg/ml pm with 0.5ug/ml Cd.

From the Gorovsky lab, University of Rochester.
Research Resource IdentifierTSC_SD02234
Requires MTA?No
Academic cost $45