Skip to main content

Follow @TetrahymenaSC

Stock IDSD02226
Strain NameATU5A-2
SpeciesT. thermophila
Genetic Descriptionatu1-1[Δ::neo1]/atu1-1[Δ::neo1]; mpr1-1/mpr1-1 or MPR1/MPR1 (atu1-1[Δ::neo1]/atu1[E441A, E443A, E445A,E446A,E447A ]; pm-r, mp-?, ?)
MAC Knockins/outsATU1 with E441A, E443A, E445A,E446A,E447A or neo replacing coding of some ATU1 genes
MIC Knockins/outsneo replaces ATU1 coding
Gene of InterestATU1 (TTHERM_00558620)
OriginJianming Duan
PublicationsHai B and Gorovsky MA. 1997. Germ-line knockout heterokaryons of an essential alpha-tubulin gene enable high-frequency gene replacement and a test of gene transfer from somatic to germ-line nuclei in tetrahymena thermophila. Proc Natl Acad Sci U S A 94(4):1310-5.

Xia L, Hai B, Gao Y, Burnette D, Thazhath R, Duan J, Bre MH, Levilliers N, Gorovsky MA, Gaertig J. 2000. Polyglycylation of tubulin is essential and affects cell motility and division in tetrahymena thermophila. J Cell Biol 149(5):1097-6.
CommentsIt is a progeny of conjugants of AAK02.7XAAKO5.5 transformed with pATU5A derived from pTUB100E3-PvuII (linearized)where the ATU1 gene is marked with Hae III and Pvu II sites and E441A, E443A, E445A,E446A,E447A. Slow growth, slow swimming, cytokinesis arrest.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD02226
Requires MTA?No
Academic cost $45