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Stock IDSD02224
Strain NameA5 (c2)
AKA
SpeciesT. thermophila
Genetic Descriptionmpr1-1/mpr1-1 (hho1[3’neo2,T35A,S43A,S45A,T47A, T54A]; pm-r, VII)
MAC Knockins/outsChange Threonine 35,47,54 and serine 43,45 to alanines
MIC Knockins/outs
Gene of InterestHHO1 (TTHERM_00823720)
OriginWei Maio
PublicationsDou Y and Gorovsky MA. 2000. Phosphorylation of linker histone H1 regulates gene expression in vivo by creating a charge patch. Mol Cell 6(2):225-31.

Dou Y, Mizzen CA, Abrams M, Allis CD, Gorovsky MA. 1999. Phosphorylation of linker histone H1 regulates gene expression in vivo by mimicking H1 removal. Mol Cell 4(4):641-7.
CommentsComplete somatic tranformant of CU428 cells with pHHOA5 /neo construct. Phosphorylation sites 1- 5 of H1 changed to alanine, neo 2 cassette in 3’flanking region for selection.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD02224
Requires MTA?No
Academic cost $35