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Stock IDSD02223
Strain NameA5 (a2)
AKA
SpeciesT. thermophila
Genetic Descriptionmpr1-1/mpr1-1 (hho1[3’neo2,T35A,S43A,S45A,T47A, T54A]; pm-r, VII)
MAC Knockins/outsChange Threonine 35,47,54 and serine 43,45 to alanines
MIC Knockins/outs
Gene of InterestHHO1 (TTHERM_00823720)
OriginWei Maio
PublicationsDou Y and Gorovsky MA. 2000. Phosphorylation of linker histone H1 regulates gene expression in vivo by creating a charge patch. Mol Cell 6(2):225-31.

Dou Y, Mizzen CA, Abrams M, Allis CD, Gorovsky MA. 1999. Phosphorylation of linker histone H1 regulates gene expression in vivo by mimicking H1 removal. Mol Cell 4(4):641-7.
CommentsComplete somatic tranformant of CU428 cells with pHHOA5 /neo construct. Phosphorylation sites 1- 5 of H1 changed to alanine, neo cassette in 3’flanking region for selection.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD02223
Requires MTA?No
Academic cost $35