Stock ID | SD02218 |
Strain Name | 5Q-GFP |
AKA | |
Species | T. thermophila |
Genetic Description | mpr1-1/mpr1-1 (hho1[3’neo2, (T35A,S43A,S45A,T47A, T54A, K29Q,K60Q,K87Q,K117Q,K150Q), GFPc]; pm-r, VII) |
MAC Knockins/outs | Change Threonine 35,47,54 and serine 43,45 to alanine (charge patch) and 5 other sites to glutamine,GFP at c-terminus |
MIC Knockins/outs | |
Gene of Interest | HHO1 (TTHERM_00823720) |
Origin | Yali Dou |
Publications | Dou Y and Gorovsky MA. 2002. Regulation of transcription by H1 phosphorylation in tetrahymena is position independent and requires clustered sites. Proc Natl Acad Sci U S A 99(9):6142-6. Dou Y and Gorovsky MA. 2000. Phosphorylation of linker histone H1 regulates gene expression in vivo by creating a charge patch. Mol Cell 6(2):225-31. |
Comments | Complete somatic tranformant of CU428 cells with p5Q /neo construct. Phosphorylation sites 1- 5 of H1 changed to alanines and 5 other sites changed to glutamines,GFP at c-terminus, neo 2 cassette in 3’ flanking region for selection. See paper From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed. |
Research Resource Identifier | TSC_SD02218 |
Requires MTA? | No |
Academic cost | $45 |