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We are now shipping live cells, plasmids, genomic DNA, and media packets, although shipping may take somewhat longer than normal. Contact us with any questions.

Stock IDSD02217
Strain Name5K-GFP
SpeciesT. thermophila
Genetic Descriptionmpr1-1/mpr1-1 (hho1[3’neo2,( T35E, S43E, S45E, T47E, T54E, A27K,T59K, S90K,V120K,A149K), GFPc]; pm-r, VII)
MAC Knockins/outsChange Threonine 35,47,54 and serine 43,45 to glutamic acid (charge patch) and 5 other sites to lysine with GFP tag
MIC Knockins/outs
Gene of InterestHHO1 (TTHERM_00823720)
OriginYali Dou
PublicationsDou Y and Gorovsky MA. 2002. Regulation of transcription by H1 phosphorylation in tetrahymena is position independent and requires clustered sites. Proc Natl Acad Sci U S A 99(9):6142-6.

Dou Y and Gorovsky MA. 2000. Phosphorylation of linker histone H1 regulates gene expression in vivo by creating a charge patch. Mol Cell 6(2):225-31.
CommentsComplete somatic tranformant of CU428 cells with pHHO5K /neo construct. Phosphorylation sites 1- 5 of H1 changed to glutamic acid and 5 other sites changed to lysines,c-terminal GFP tag neo 2 cassette in 3’ flanking region for selection. See paper.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD02217
Requires MTA?No
Academic cost $45