Tetrahymena Stock Center| Stock ID | SD02203 |
| Strain Name | YN-HTAX (9) |
| AKA | |
| Species | T. thermophila |
| Genetic Description | mpr1-1/mpr1-1 (HTAY/htay[∆ ::neo3]; MTT1/mtt1[5’neo2, ∆ :: 2HAn,(HTAY1-258, HTAX1-138)]; pm-r, VII) |
| MAC Knockins/outs | Neo3 into HTAY, Chimeric YN-HTAX into MTT1 locus |
| MIC Knockins/outs | |
| Gene of Interest | HTAY (TTHERM_01079200) and HTA1 (TTHERM_00790790) |
| Origin | Jody Bowen |
| Publications | Song X, Bowen J, Miao W, Liu Y, Gorovsky MA. 2012. The nonhistone, N-terminal tail of an essential, chimeric H2A variant regulates mitotic H3-S10 dephosphorylation. Genes Dev 26(6):615-29. |
| Comments | Shot Xiaoyuan Song’s i∆HTAY strain(partial replacement of HTAY with neo3) with construct MTT1-2HA tags after the ATG of HTAY n-terminus (AA1-258) followed by HTAX AA1-138) also has neo2 in 5’ flank of MTT1.Single cell line (5B) with most or all of HTAY replaced by the HA tagged YN-HTAX. Pushed to 30mg/ml pm resistance with Cd at 0.1-.0.5 ug/ml needed to express the YN-HTAX From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed. |
| Research Resource Identifier | TSC_SD02203 |
| Requires MTA? | No |
| Academic cost | $45 |
