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We are now shipping live cells, plasmids, genomic DNA, and media packets, although shipping may take somewhat longer than normal. Contact us with any questions.

Stock IDSD02200
Strain NameYN-HTAX (1)
SpeciesT. thermophila
Genetic Descriptionmpr1-1/mpr1-1 (HTAY/htay[∆ ::neo3]; MTT1/mtt1[5’neo2, ∆ :: 2HAn,(HTAY1-258, HTAX1-138)]; pm-r, VII)
MAC Knockins/outsNeo3 into HTAY, Chimeric YN-HTAX into MTT1 locus
MIC Knockins/outs
Gene of InterestHTAY (TTHERM_01079200) and HTA1 (TTHERM_00790790)
OriginJody Bowen
PublicationsSong X, Bowen J, Miao W, Liu Y, Gorovsky MA. 2012. The nonhistone, N-terminal tail of an essential, chimeric H2A variant regulates mitotic H3-S10 dephosphorylation. Genes Dev 26(6):615-29.
CommentsShot Xiaoyuan Song’s i∆HTAY strain (partial replacement of HTAY with neo3) with construct MTT1-2HA tags after the ATG of HTAY n-terminus (AA1-258) followed by HTAX AA1-138) also has neo2 in 5’ flank of MTT1.Single cell line (1A) with most or all of HTAY replaced by the HA tagged YN-HTAX.

Pushed to 30mg/ml pm resistance with Cd at 0.1-.0.5 ug/ml needed to express the YN-HTAX.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD02200
Requires MTA?No
Academic cost $45