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Stock IDSD02192
Strain NameTYA (@ GTU1 locus ) #5
AKA
SpeciesT. thermophila
Genetic Descriptiongtu1[∆::neo2]/ gtu1[∆::neo2] (gtu1[∆::neo2] /gtu1[GTU1,T270A,Y272A ]; pm-r, ?)
MAC Knockins/outsGTU1 with mutation T270A,Y272A. Not sure if complete replacement.
MIC Knockins/outsNeo2 into GTU1 locus
Gene of InterestGTU1 (TTHERM_00079520)
OriginYuhua Shang
PublicationsShang Y, Li B, Gorovsky MA. 2002. Tetrahymena thermophila contains a conventional gamma-tubulin that is differentially required for the maintenance of different microtubule-organizing centers. J Cell Biol 158(7):1195-206.

Shang Y, Tsao CC, Gorovsky MA. 2005. Mutational analyses reveal a novel function of the nucleotide-binding domain of gamma-tubulin in the regulation of basal body biogenesis. J Cell Biol 171(6):1035-44.
CommentspT270Y272 rescued GTUKO 5x6 progeny having mutation T270A,Y272A in GTU1 in GTU1 locus Cell line #5 can be crossed with cell line #6.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD02192
Requires MTA?No
Academic cost $35