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Stock IDSD02167
Strain NameHTA1+HTB1-E4a
AKA
SpeciesT. thermophila
Genetic Descriptionhtax[∆::neo2]/htax[∆::neo2];hta1[∆::neo2]/hta1[∆::neo2] ; htb1[∆::neo2]/htb1[∆::neo2];htb2[∆::neo2]/htb2[∆::neo2] (HTAX/htax[∆neo2];hta1[∆::neo2]; HTB1/htb1[∆::neo2]; htb2[∆::neo2]; pm-r, ?)
MAC Knockins/outsWT HTAX and HTB1, neo2 of HTA1,HTB2
MIC Knockins/outsneo2 of HTAX,HTA1,HTB1,HTB2
Gene of InterestHTA1 (TTHERM_00790790), HTA2 (TTHERM_00316500), HTB1 (TTHERM_00633360), HTB2 (TTHERM_00283180)
OriginZhe Wang
PublicationsWang Z, Cui B, Gorovsky MA. 2009. Histone H2B ubiquitylation is not required for histone H3 methylation at lysine 4 in tetrahymena. J Biol Chem 284(50):34870-9.

Wang Z. 2007. Functional studies of histone ubiquitylation in tetrahymena thermophila. University of Rochester.
CommentsHTX1/HTB1 double wt rescue; Rescue of h∆∆HTA∆∆HTB-FBD1F6 and FBB1D3 with Dryl's.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD02167
Requires MTA?No
Academic cost $35