Skip to main content

Follow @TetrahymenaSC

We are now shipping live cells, plasmids, genomic DNA, and media packets, although shipping may take somewhat longer than normal. Contact us with any questions.

Stock IDSD02164
Strain Namehta1-K123R+htb1-K115R-E1a
SpeciesT. thermophila
Genetic Descriptionhtax[∆::neo2]/htax[∆::neo2];hta1[∆::neo2]/hta1[∆::neo2] ; htb1[∆::neo2]/htb1[∆::neo2];htb2[∆::neo2]/htb2[∆::neo2] (pm-r, ?)
MAC Knockins/outsHTAX with K123R and HTB1 with K115R, neo2 of HTA1,HTB2
MIC Knockins/outsneo2 of HTAX,HTA1,HTB1,HTB2
Gene of InterestHTA1 (TTHERM_00790790), HTA2 (TTHERM_00316500), HTB1 (TTHERM_00633360), HTB2 (TTHERM_00283180)
OriginZhe Wang
PublicationsWang Z, Cui B, Gorovsky MA. 2009. Histone H2B ubiquitylation is not required for histone H3 methylation at lysine 4 in tetrahymena. J Biol Chem 284(50):34870-9.

Wang Z. 2007. Functional studies of histone ubiquitylation in tetrahymena thermophila. University of Rochester.
CommentsHTX1/HTB1 double mutant rescue; Rescue of h∆∆HTA∆∆HTB-FBD1F6 and FBB1D3 with Dryl's.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD02164
Requires MTA?No
Academic cost $45