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Stock IDSD02143
Strain NameGTU-TAP (1)
AKA
SpeciesT. thermophila
Genetic Descriptiongtu1[∆::neo2]/ gtu1[∆::neo2] (gtu1[∆::neo2]; MTT1/mtt1[∆, (::GTU-HA-TAP]; pm-r, ?)
MAC Knockins/outsInto MTT1 locus, GTU1 with HA and TAP tag
MIC Knockins/outsneo2
Gene of InterestGTU1 (TTHERM_00079520)
OriginYuhua Shang
PublicationsShang Y, Li B, Gorovsky MA. 2002. Tetrahymena thermophila contains a conventional gamma-tubulin that is differentially required for the maintenance of different microtubule-organizing centers. J Cell Biol 158(7):1195-206.
CommentsRescue of GTU05XGTU06 knockout heterokaryons with pTTMG-HA TAP. GTU1 with HA+TAP tag into MTT1 locus. MTT1 promoter. Grow with 0.2ug/ml CdCl in 1xSPP.

5’MTT1-GTU-HA-TAP-MTT1 3’
TAP tag from Eric Phzicky is:
GTU-HA-6XHis-3Cprotease cleavage-S.Aureus protein A. Constuct pTTMG-HA used to rescue GTU5 xGTU06 KO heterokaryons.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD02143
Requires MTA?No
Academic cost $35