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Stock IDSD02142
Strain NamegDRH1-KO4 D3-1B
SpeciesT. thermophila
Genetic Descriptiondrh1[∆?::neo4]/drh1[∆?::neo4]
MAC Knockins/outs
MIC Knockins/outsNeo4 into 5’ end of coding of DRH1
Gene of InterestDRH1 (TTHERM_00190830)
OriginJody Bowen
CommentsNeo4 KO cassette in DRH1 locus, homozygous KO in mic, mac assorted to pm-s. Two cell lines in row. Mate with A5, H9, and C10 cell lines. Homozygous heterokaryon.

Gene is expressed at low level in starve and log, peak expression at 4 hr of conjugation. Somatic KO is complete, when mated they arrest after Meiosis II. Homozygous homokaryon KOs do not grow well.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD02142
Requires MTA?No
Academic cost $45