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We are now shipping live cells, plasmids, genomic DNA, and media packets, although shipping may take somewhat longer than normal. Contact us with any questions.

Stock IDSD02122
Strain Name1235D-1(BTU1)
SpeciesT. thermophila
Genetic Descriptionbtu1-?[∆::neo1]/ btu1-?[∆::neo1]; btu2-?[∆::bsr1]/ btu2-?[∆::bsr1]; CHX1/CHX1; mpr1-1/mpr1-1 or MPR1/MPR1 (btu2-?[∆::bsr1]; btu1-?[∆::neo1]/ btu1-? [E431D,E432D,E433D,E435D]; pm-r)
MAC Knockins/outsBTU1 has only (E431,432,433,435D) no WT
MIC Knockins/outsknockout of btu1 with neo
knockout of btu2 with bsr
Gene of InterestBTU1 (TTHERM_00348510), BTU2 (TTHERM_00836580)
OriginJianming Duan
PublicationsXia L, Hai B, Gao Y, Burnette D, Thazhath R, Duan J, Bre MH, Levilliers N, Gorovsky MA, Gaertig J. 2000. Polyglycylation of tubulin is essential and affects cell motility and division in tetrahymena thermophila. J Cell Biol 149(5):1097-6.
CommentsProgeny of DB6B x DB2A, transformed by BTU1-1235D mutation construct (E431,432,433,435D)

From the Gorovsky lab, University of Rochester.
Research Resource IdentifierTSC_SD02122
Requires MTA?No
Academic cost $45