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We are now shipping live cells, plasmids, genomic DNA, and media packets, although shipping may take somewhat longer than normal. Contact us with any questions.

Stock IDSD02113
Strain NameTWI1-soma KO4
SpeciesT. thermophila
Genetic Description(Mac∆TWI1::neo3, twi1[∆::neo3]; mpr1-1; pm-r, mp-r, ?)
MAC Knockins/outsCoding region of TWI1 replaced by neo3
MIC Knockins/outs
Gene of InterestTWI1 (TTHERM_01161040)
OriginKazufumi Mochizuki
PublicationsMochizuki K, Fine NA, Fujisawa T, Gorovsky MA. 2002. Analysis of a piwi-related gene implicates small RNAs in genome rearrangement in tetrahymena. Cell 110(6):689-99.
CommentsB2086 X CU428 were transformed with TWI1 KO construct (whole coding was replaced by MTT-neo-BTU) and endogenous TWI1 gene was pushed out. Somatic transformant
this strain can mate with B2086, cu427, TWI1-KO2,-KO10.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD02113
Requires MTA?No
Academic cost $45