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Stock IDSD02069
Strain NameHA-TWI1-1-1
SpeciesT. thermophila
Genetic Descriptionmpr1-1/mpr1-1 (twi1[HAn,3’neo3]; TWI1::HA-TWi1-neo3, pm-r, VII)
MAC Knockins/outsN-terminal HA tag on TWI1 with 3’ flanking neo3
MIC Knockins/outs
Gene of InterestTWI1 (TTHERM_01161040)
OriginKazufumi Mochizuki
PublicationsMochizuki K, Fine NA, Fujisawa T, Gorovsky MA. 2002. Analysis of a piwi-related gene implicates small RNAs in genome rearrangement in tetrahymena. Cell 110(6):689-99.
CommentsCU428 was transformed with HA_TWI1-neo3 and endogenous TWI1 loci were completely replaced. See Mochizuki et al (2002) Cell 110,689-699. Chaker and Allis labs have some strains.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD02069
Requires MTA?No
Academic cost $35