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Stock IDSD02060
Strain Nameh∆∆HTA∆∆HTB-FBD1G3
SpeciesT. thermophila
Genetic Descriptionhtax[∆::neo2]/htax[∆::neo2];hta1[∆::neo2]/hta1[∆::neo2] ; htb1[∆::neo2]/htb1[∆::neo2];htb2[∆::neo2]/htb2[∆::neo2 (mp-r?, II)
MAC Knockins/outs
MIC Knockins/outsneo2 replaces coding of HTAX,HTA1,HTB1,HTB2
Gene of InterestHTA1 (TTHERM_00790790), HTA2 (TTHERM_00316500), HTB1 (TTHERM_00633360), HTB2 (TTHERM_00283180)
OriginZhe Wang
PublicationsWang Z. 2007. Functional studies of histone ubiquitylation in tetrahymena thermophila. University of Rochester.

Wang Z, Cui B, Gorovsky MA. 2009. Histone H2B ubiquitylation is not required for histone H3 methylation at lysine 4 in tetrahymena. J Biol Chem 284(50):34870-9.
CommentsHTX1/HTB1 quadruple KO heterokaryons;From mating of h∆∆HTA-F14A and h∆∆HTB-B3D3; made with B*VII.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD02060
Requires MTA?No
Academic cost $35