Skip to main content


Follow @TetrahymenaSC

Stock IDSD02043
Strain NameA101GHA #1
AKA
SpeciesT. thermophila
Genetic Descriptiongtu1[∆::neo2]/ gtu1[∆::neo2] (gtu1[∆::neo2]; MTT1/mtt1[∆, HAc(::GTU1, A101G)]; pm-r, ?)
MAC Knockins/outsGTU1 with mutations A101G and c-terminus HA tag into the MTT1 locus, partial replacement.
MIC Knockins/outsNeo2 into GTU1 locus
Gene of InterestGTU1 (TTHERM_00079520)
OriginYuhua Shang
PublicationsShang Y, Tsao CC, Gorovsky MA. 2005. Mutational analyses reveal a novel function of the nucleotide-binding domain of gamma-tubulin in the regulation of basal body biogenesis. J Cell Biol 171(6):1035-44.

Shang Y, Li B, Gorovsky MA. 2002. Tetrahymena thermophila contains a conventional gamma-tubulin that is differentially required for the maintenance of different microtubule-organizing centers. J Cell Biol 158(7):1195-206.
CommentspGTUA101G-HA rescued GTUKO 5x6 progeny having mutation @ GTU1 A101G in GTU1-1 gene, driven by MTT1 promoter.

Grow and maintain in 0.5 ug/ml CdCl2 at 30C.

From the Gorovsky lab, University of Rochester.
Research Resource IdentifierTSC_SD02043
Requires MTA?No
Academic cost $35