Stock ID | SD02016 |
Strain Name | ∆∆DB+BTU1(3) |
AKA | |
Species | T. thermophila |
Genetic Description | btu1-?[∆::neo1]/ btu1-?[∆::neo1]; btu2-?[∆::bsr1]/ btu2-?[∆::bsr1] (BTU1/btu1-?[∆::neo1];btu2-?[∆::bsr1] not sure if completely assorted to WT BTU1; No BTU2 gene product, blasticidin resistant. WT BTU1 gene replaced ∆BTU1. Not sure if assorted to pm -s yet., ?) |
MAC Knockins/outs | BTU1 into mac, still btu2 KO with bsr |
MIC Knockins/outs | BTU1 coding replaced by neo1 BTU2 coding replaced by bsr1 |
Gene of Interest | BTU1 (TTHERM_00348510) + BTU2 (TTHERM_00836580) |
Origin | Jody Bowen |
Publications | |
Comments | Mated Jacek Gaertig’s double BTU1/BTU2 KO heterokaryons, DB2A x DB6B, rescue with BTU1. Same as ∆∆DB+BTU1(2). Separate cell line. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed. |
Research Resource Identifier | TSC_SD02016 |
Requires MTA? | No |
Academic cost | $45 |