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Stock IDSD02012
Strain NameDB2Apm-3
SpeciesT. thermophila
Genetic Descriptionbtu1[∆,::neo1]/btu1[∆,::neo1]; btu2[∆,::bsr1]/btu2[∆,::bsr1] (assorted to pm-s so BTU1 assorted to bsr-s so BTU2; mp-r, ?)
MAC Knockins/outsassorted to WT or lost resistance cassettes
MIC Knockins/outsBTU1 coding replaced with neo1 cassette; BTU2 is blasticidin KO.
Gene of InterestBTU1 (TTHERM_00348510) + BTU2 (TTHERM_00836580)
OriginJacek Gaertig, frozen by Jianming Duan
PublicationsXia L, Hai B, Gao Y, Burnette D, Thazhath R, Duan J, Bre MH, Levilliers N, Gorovsky MA, Gaertig J. 2000. Polyglycylation of tubulin is essential and affects cell motility and division in tetrahymena thermophila. J Cell Biol 149(5):1097-6.
CommentsObtained from Jacek Gaertig, Jianming single celled. Germline KO BTU1 neo1 cassette; BTU2 is blasticidin KO. Mates with DB6Bpm-1.-2,-3 for heterokaryon rescue.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD02012
Requires MTA?No
Academic cost $45