Tetrahymena Stock Center| Stock ID | SD02010 |
| Strain Name | DB2Apm-1 |
| AKA | |
| Species | T. thermophila |
| Genetic Description | btu1[∆,::neo1]/btu1[∆,::neo1]; btu2[∆,::bsr1]/btu2[∆,::bsr1] (assorted to pm-s so BTU1 assorted to bsr-s so BTU2; mp-r, ?) |
| MAC Knockins/outs | assorted to WT or lost resistance cassettes |
| MIC Knockins/outs | BTU1 coding replaced with neo1 cassette; BTU2 is blasticidin KO. |
| Gene of Interest | BTU1 (TTHERM_00348510) + BTU2 (TTHERM_00836580) |
| Origin | Jacek Gaertig, frozen by Jianming Duan |
| Publications | Xia L, Hai B, Gao Y, Burnette D, Thazhath R, Duan J, Bre MH, Levilliers N, Gorovsky MA, Gaertig J. 2000. Polyglycylation of tubulin is essential and affects cell motility and division in tetrahymena thermophila. J Cell Biol 149(5):1097-6. |
| Comments | Obtained from Jacek Gaertig, Jianming single celled. Germline KO BTU1 neo1 cassette; BTU2 is blasticidin KO. Mates with DB6Bpm-1,-2,-3 for heterokaryon rescue. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed. |
| Research Resource Identifier | TSC_SD02010 |
| Requires MTA? | No |
| Academic cost | $45 |
