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We are now shipping live cells, plasmids, genomic DNA, and media packets, although shipping may take somewhat longer than normal. Contact us with any questions.

Stock IDSD01998
Strain Name∆∆DB+BTU1(2)
SpeciesT. thermophila
Genetic Descriptionbtu1-?[∆::neo1]/ btu1-?[∆::neo1]; btu2-?[∆::bsr1]/ btu2-?[∆::bsr1] (BTU1/btu1-?[∆::neo1];btu2-?[∆::bsr1] not sure if completely assorted to WT BTU1; No BTU2 gene product, blasticidin resistant. WT BTU1 gene replaced ∆BTU1. Not sure if assorted to pm-s yet., ?)
MAC Knockins/outsBTU1 into mac, still btu2 KO with bsr
MIC Knockins/outsBTU1 coding replaced by neo1
BTU2 coding replaced by bsr1
Gene of InterestBTU1 (TTHERM_00348510) + BTU2 (TTHERM_00836580)
OriginJody Bowen
CommentsMated Jacek Gaertig’s double BTU1/BTU2 KO heterokaryons, DB2A x DB6B, rescue with BTU1 . Same as ∆∆DB+BTU1(3). Separate cell line.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD01998
Requires MTA?No
Academic cost $45