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We are now shipping live cells, plasmids, genomic DNA, and media packets, although shipping may take somewhat longer than normal. Contact us with any questions.

Stock IDSD01997
Strain Name∆HTAY pXS83-33
SpeciesT. thermophila
Genetic Description(HTAY?/htay[∆::neo3]; MTT1/mtt1[5’neo2, ∆, 2HAn(::HTAY)]; pm-r, ?)
MAC Knockins/outsNeo3 KO of HTAY (may be incomplete) and HTAY with two HA tags on the N-terminus inserted into MTT1 locus with neo2 cassete in 5’ flanking region
MIC Knockins/outs
Gene of InterestH2A.Y (TTHERM_01079200)
OriginXiaoyuan Song
PublicationsSong X, Bowen J, Miao W, Liu Y, Gorovsky MA. 2012. The nonhistone, N-terminal tail of an essential, chimeric H2A variant regulates mitotic H3-S10 dephosphorylation. Genes Dev 26(6):615-29.
CommentsTransformed pXS83 (MTT-neo2-2HA-HTAY-MTT) into HTA.Y real (true, longer coding seq.) somatic knockout strain in MTT1 locus. Selected in the presence of Cd +Pm. PCR shows very faint band of WT HTA.Y. Same as pXS83-23.

Grow in 100ug/ml pm, 0.25 ug/ml CdCl2.

From the Gorovsky lab, University of Rochester. This strain has been catalogued by the Stock Center, but not thawed.
Research Resource IdentifierTSC_SD01997
Requires MTA?No
Academic cost $45