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We are now shipping live cells, plasmids, genomic DNA, and media packets, although shipping may take somewhat longer than normal. Contact us with any questions.

Stock IDSD01995
Strain Name∆H3 all, gl KO, M7-3
SpeciesT. thermophila
Genetic Descriptionhht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆::neo2]/hhf2,hht2[∆::neo2]; hht3[∆::neo2]/hht3[∆::neo2]; mpr1-1/mpr1-1 (pm-s, mp-r, wt HHT1,2,3 HHF2, VII)
MAC Knockins/outs
MIC Knockins/outsNeo3 KO of HHT!,HHT2/HHF2,HHT3
Gene of InterestHHT + HHF (TTHERM_00570560, TTHERM_00189180, TTHERM_00189170)
PublicationsLiu Y, Song X, Gorovsky MA, Karrer KM. 2005. Elimination of foreign DNA during somatic differentiation in tetrahymena thermophila shows position effect and is dosage dependent. Eukaryot Cell 4(2):421-31.

Liu Y. 2003. Genetic studies of RNA-mediated DNA elimination in the developing macronuclei of tetrahymena thermophila. University of Rochester.
CommentsGermline heteokaryon for all three H3 genes(major and minor) and H4II, neo cassette. Note: now know there is a HHT4 which was still present

From the Gorovsky lab, University of Rochester.
Research Resource IdentifierTSC_SD01995
Requires MTA?No
Academic cost $45