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We are now shipping live cells, plasmids, genomic DNA, and media packets, although shipping may take somewhat longer than normal. Contact us with any questions.

Stock IDSD01960
Strain Name∆H3 gl 10.5
SpeciesT. thermophila
Genetic Descriptionhht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆::neo2]/hhf2,hht2[∆::neo2]; mpr1-1/mpr1-1 (mpr1-1; mp-r, ?)
MAC Knockins/outs
MIC Knockins/outsNeo2 ko of HHT1 and HHT2/HHF2
Gene of InterestHHT2 (TTHERM_00189180), HHF2 (TTHERM_00189170)
OriginYifan Liu
PublicationsCui B, Liu Y, Gorovsky MA. 2006. Deposition and function of histone H3 variants in Tetrahymena thermophila. Mol Cell Biol. 26:7719-30.
CommentsThis is the germline KO homozygous heterokaryon. Both copies of major histone H3 were removed. Also HHF2 and intergenic region connecting it to HHT2. KO with Neo cassette. The ∆H3 gl 2.* series have a different mating type from the ∆H3 gl 10.* series All the 10.* series are separate cell lines.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD01960
Requires MTA?No
Academic cost $45