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We are now shipping live cells, plasmids, genomic DNA, and media packets, although shipping may take somewhat longer than normal. Contact us with any questions.


Stock IDSD01953
Strain Nameg:H3.3+GFP/H3.2-54
AKA
SpeciesT. thermophila
Genetic Descriptionhht2[3’neo2, ∆::(HHT3,GFPc)]/hht2[3’neo2, ∆::(HHT3,GFPc)] (?HHT2/hht2[3’neo2 ∆::(HHT3,GFPc)]; ?)
MAC Knockins/outs
MIC Knockins/outsC-terminal GFP- tag of HHT3, into HHT2 locus
Gene of InterestHTT3 (TTHERM_00016170), HHT2 (TTHERM_00189180)
OriginBowen Cui
PublicationsCui B, Liu Y, Gorovsky MA. 2006. Deposition and function of histone H3 variants in tetrahymena thermophila. Mol Cell Biol 26(20):7719-30.
Commentsgermline H3.3 +GFP at H3.2, heterokaryon. lnon-star sideof H3.3+GFP germline replacement at H3.2 locus.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD01953
Requires MTA?No
Academic cost $45