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We are now shipping live cells, plasmids, genomic DNA, and media packets, although shipping may take somewhat longer than normal. Contact us with any questions.

Stock IDSD01942
Strain NameDB2A
SpeciesT. thermophila
Genetic Descriptionbtu1[∆,::neo1]/btu1[∆,::neo1]; btu2[∆,::bsr1]/btu2[∆,::bsr1] (BTU1/btu1[∆,::neo1], BTU2; bsr-s, pm-r, ?)
MAC Knockins/outssome BTU1::neo1 genes
MIC Knockins/outsBTU1 coding replaced with neo1 cassette; BTU2 is blasticidin KO.
Gene of InterestBTU1 (TTHERM_00348510) and BTU2 (TTHERM_00836580)
OriginJacek Gaertig
PublicationsXia L, Hai B, Gao Y, Burnette D, Thazhath R, Duan J, Bre MH, Levilliers N, Gorovsky MA, Gaertig J. 2000. Polyglycylation of tubulin is essential and affects cell motility and division in tetrahymena thermophila. J Cell Biol 149(5):1097-6.
CommentsObtained from Jacek 11/1/99. Germline KO BTU1 neo1 cassette; BTU2 is blasticidin KO.

Mates with DB6B for heterokaryon rescue.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD01942
Requires MTA?No
Academic cost $45