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Stock IDSD01913
Strain NameBTU1-E431-3, 435D
AKA
SpeciesT. thermophila
Genetic Descriptionbtu1-?[∆::neo1]/ btu1-?[∆::neo1]; btu2-?[∆::bsr1]/ btu2-?[∆::bsr1]; CHX1/CHX1; mpr1-1/mpr1-1 or MPR1/MPR1 (btu2-?[∆::bsr1]; btu1-?[∆::neo1]/ btu1-? [E431D,E432D,E433D,E435D]; pm-r, ?)
MAC Knockins/outsBTU1 has only (E431,432,433,435D) no WT
BTU2 is KO
MIC Knockins/outsknockout of btu1 with neo
knockout of btu2 with bsr
Gene of InterestBTU1 (TTHERM_00348510), BTU2 (TTHERM_00836580)
OriginJianming Duan
PublicationsXia L, Hai B, Gao Y, Burnette D, Thazhath R, Duan J, Bre MH, Levilliers N, Gorovsky MA, Gaertig J. 2000. Polyglycylation of tubulin is essential and affects cell motility and division in tetrahymena thermophila. J Cell Biol 149(5):1097-6.

Duan J and Gorovsky MA. 2002. Both carboxy-terminal tails of alpha- and beta-tubulin are essential, but either one will suffice. Curr Biol 12(4):313-6.
CommentsProgeny of DB6B x DB2A, transformed by BTU1-1235D mutation construct (E431,432,433,435D).

From the Gorovsky lab, University of Rochester. This strain has been thawed and is no longer paromomycin resistant.
Research Resource IdentifierTSC_SD01913
Requires MTA?No
Academic cost $35