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We are now shipping live cells, plasmids, genomic DNA, and media packets, although shipping may take somewhat longer than normal. Contact us with any questions.


Stock IDSD01912
Strain NameB2KO-8
AKA
SpeciesT. thermophila
Genetic DescriptionBTU2/btu2[∆,::H4-neo]; MPR1-1/mpr1-1 (BTU2/btu1[∆,::H4-neo]; MPR1-1/mpr1-1; mp-r, pm-r, ?)
MAC Knockins/outsH4-neo replaces BTU2 coding, not sure if complete
MIC Knockins/outsH4-neo replaces BTU2 coding
Gene of InterestBTU2 (TTHERM_00836580)
OriginBing Hai
PublicationsFor another source of ∆BTU2 see:

Xia L, Hai B, Gao Y, Burnette D, Thazhath R, Duan J, Bre MH, Levilliers N, Gorovsky MA, Gaertig J. 2000. Polyglycylation of tubulin is essential and affects cell motility and division in tetrahymena thermophila. J Cell Biol 149(5):1097-6.
CommentsHeterozygous germline transformant of BTU2 knockout. Derived from Cu428 X B2086 transformed w/ BHAB-2 (construct). When crossed to CU427, gave cyr and pmr progeny.
Can mate to B2KO-6. Created 12/29/97, in soybean until 4/98.

B2KO-6 mates with B2KO-1,2,3,4,5,7,8. No others mated.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD01912
Requires MTA?No
Academic cost $45