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We are now shipping live cells, plasmids, genomic DNA, and media packets, although shipping may take somewhat longer than normal. Contact us with any questions.

Stock IDSD01898
Strain NameS-H3.2+GFP/H3.3-422
SpeciesT. thermophila
Genetic Descriptionmpr1-1/mpr1-1 (HHT3?/htt3[5’neo2, ∆, (::HHT2,GFPc)]; pm-r, VII)
MAC Knockins/outsHHT2 with GFP c-terminal tag replaces the HHT3 coding region
MIC Knockins/outs
Gene of InterestHHT2 (TTHERM_00189180), HHT3 (TTHERM_00016170)
OriginBowen Cui
PublicationsCui B, Liu Y, Gorovsky MA. 2006. Deposition and function of histone H3 variants in tetrahymena thermophila. Mol Cell Biol 26(20):7719-30.
Commentssomatic replace H3.3 coding with H3.2+GFP, neo 2 cassette in 5’ flank of H3.3. Not sure if complete replacement.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD01898
Requires MTA?No
Academic cost $45