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We have resumed shipping live cells and will soon ship plasmids and extracted DNA. Transformation services will take longer to resume.

Stock IDSD01896
Strain NameS-H3.3+GFP/H3.2-8
SpeciesT. thermophila
Genetic Descriptionmpr1-1/mpr1-1 (?HHT2/hht2[3’neo2 ∆::(HHT3,GFPc)]; pm-r, VII)
MAC Knockins/outsC-terminal GFP-tag of HHT3, not sure if complete replacement, into HHT2 locus
MIC Knockins/outs
Gene of InterestHTT3 (TTHERM_00016170)
OriginBowen Cui
PublicationsCui B, Liu Y, Gorovsky MA. 2006. Deposition and function of histone H3 variants in tetrahymena thermophila. Mol Cell Biol 26(20):7719-30.
CommentsSomatic replace HHT2 coding region with HHT3-GFP sequence. Not sure where the neo2 cassette is.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD01896
Requires MTA?No
Academic cost $45