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We are now shipping live cells, plasmids, genomic DNA, and media packets, although shipping may take somewhat longer than normal. Contact us with any questions.

Stock IDSD01894
Strain NameHHT2-S10 (S10-4 3)
SpeciesT. thermophila
Genetic Descriptionmpr1-1/mpr1-1 (hht1[∆::neo2] ; hht2[3’neo2,S10A]; pm-r, VII)
MAC Knockins/outsHHT1 replaced by neo2, HHT2 has S10A mutation
MIC Knockins/outs
Gene of InterestHHT2 (TTHERM_00189180), HHT1
OriginLanlan Yu
PublicationsWei Y, Mizzen CA, Cook RG, Gorovsky MA, Allis CD. 1998. Phosphorylation of histone H3 at serine 10 is correlated with chromosome condensation during mitosis and meiosis in tetrahymena. Proc Natl Acad Sci U S A 95(13):7480-4.

Wei Y, Yu L, Bowen J, Gorovsky MA, Allis CD. 1999. Phosphorylation of histone H3 is required for proper chromosome condensation and segregation. Cell 97(1):99-109.
CommentsSerine 10 was mutated to Ala in the coding region of the HHT2 gene. Then the mutated HHT2 gene with its flanking neo marker was transformed into ∆HHT1. Southern blot indicated it is a complete replacement.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD01894
Requires MTA?No
Academic cost $45