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We are now shipping live cells, plasmids, genomic DNA, and media packets, although shipping may take somewhat longer than normal. Contact us with any questions.

Stock IDSD01889
Strain NameH3E-6
SpeciesT. thermophila
Genetic Descriptionchx1-1/chx1-1 (?HHT1/hht1[∆::neo2; hht2[3’neo2, S10A]; Somatic ∆HHT1 with neo cassette and HHT2S10A ( neo in 3 flank) cotransformant., VI)
MAC Knockins/outsKO of HHT1, HHT2 has mutation S10A
MIC Knockins/outs
Gene of InterestHHT1 (TTHERM_00570560), HHT2 (TTHERM_00189180)
OriginJody Bowen
PublicationsWei Y, Mizzen CA, Cook RG, Gorovsky MA, Allis CD. 1998. Phosphorylation of histone H3 at serine 10 is correlated with chromosome condensation during mitosis and meiosis in tetrahymena. Proc Natl Acad Sci U S A 95(13):7480-4.

Wei Y, Yu L, Bowen J, Gorovsky MA, Allis CD. 1999. Phosphorylation of histone H3 is required for proper chromosome condensation and segregation. Cell 97(1):99-109.
CommentsSingle cell line from H3E transformant.
By antiphos H3 antibody staining, no stain so should be all HHT2 S10A. Also checked by Southern.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD01889
Requires MTA?No
Academic cost $45