Tetrahymena Stock Center| Stock ID | SD01848 |
| Strain Name | D5AB |
| AKA | |
| Species | T. thermophila |
| Genetic Description | btu1-?[∆::neo1]/ btu1-?[∆::neo1]; btu2-?[∆::bsr1]/ btu2-?[∆::bsr1]; CHX1/CHX1; mpr1-1/mpr1-1 or MPR1/MPR1 (btu2-?[∆::bsr1]; btu1-?[∆::neo1]/ btu1-? [E437D,E438D,E439D,E440D,E442D]; ATU1/atu1-1[α-β hybrid tail, ATU-B]; pm-r, Original BTU2 is replaced by bsr1, BTU1 coding replaced by neo1, then transformed with pBTU1-D5 (D-DDDD) and Chimeric ATU1 gene, ATU-B (α-β hybrid )) |
| MAC Knockins/outs | BTU2 replaced by bsr. BTU1 partially replaced by neo1, some replaced by BTU1-D5 with (E437-442D). Some ATU1 replaced by ATU1 with chimeric α-β hybrid tail. |
| MIC Knockins/outs | knockout of btu1 with neo knockout of btu2 with bsr |
| Gene of Interest | BTU1 (TTHERM_00348510), BTU2 (TTHERM_00836580), ATU1 (TTHERM_00558620) |
| Origin | Jianming Duan |
| Publications | Duan J and Gorovsky MA. 2002. Both carboxy-terminal tails of alpha- and beta-tubulin are essential, but either one will suffice. Curr Biol 12(4):313-6. Xia L, Hai B, Gao Y, Burnette D, Thazhath R, Duan J, Bre MH, Levilliers N, Gorovsky MA, Gaertig J. 2000. Polyglycylation of tubulin is essential and affects cell motility and division in tetrahymena thermophila. J Cell Biol 149(5):1097-6. Thazhath R, Jerka-Dziadosz M, Duan J, Wloga D, Gorovsky MA, Frankel J, Gaertig J. 2004. Cell context-specific effects of the beta-tubulin glycylation domain on assembly and size of microtubular organelles. Mol Biol Cell 15(9):4136-47. |
| Comments | Progeny of DB6B x DB2A, co- transformed by constructs pBTU1-D5 (D-DDDD) and Chimeric ATU1 gene, pATU-B (α-β hybrid). See publications From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed. |
| Research Resource Identifier | TSC_SD01848 |
| Requires MTA? | No |
| Academic cost | $45 |
