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We are now shipping live cells, plasmids, genomic DNA, and media packets, although shipping may take somewhat longer than normal. Contact us with any questions.

Stock IDSD01841
Strain NameBTU1-E431,432,435D
SpeciesT. thermophila
Genetic Descriptionbtu1-?[∆::neo1]/ btu1-?[∆::neo1]; btu2-?[∆::bsr1]/ btu2-?[∆::bsr1]; CHX1/CHX1; mpr1-1/mpr1-1 or MPR1/MPR1 (btu2-?[∆::bsr1]; btu1-?[∆::neo1]/ btu1-? [E431D,E432D,E435D])
MAC Knockins/outsBTU1 has only (E431,432,435D) no WT
BTU2 is KO
MIC Knockins/outsknockout of btu1 with neo
knockout of btu2 with bsr
Gene of InterestBTU1 (TTHERM_00348510), BTU2 (TTHERM_00836580)
OriginJianming Duan
PublicationsThazhath R, Jerka-Dziadosz M, Duan J, Wloga D, Gorovsky MA, Frankel J, Gaertig J. 2004. Cell context-specific effects of the beta-tubulin glycylation domain on assembly and size of microtubular organelles. Mol Biol Cell 15(9):4136-47.

Duan J and Gorovsky MA. 2002. Both carboxy-terminal tails of alpha- and beta-tubulin are essential, but either one will suffice. Curr Biol 12(4):313-6.
CommentsProgeny of DB6B x DB2A, transformed by BTU1-1235D mutation construct (E431,432, 435D).

The strain was supposed to be pm-r, but the thawed line is pm-s.

From the Gorovsky lab, University of Rochester.
Research Resource IdentifierTSC_SD01841
Requires MTA?No
Academic cost $45