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We are now shipping live cells, plasmids, genomic DNA, and media packets, although shipping may take somewhat longer than normal. Contact us with any questions.

Stock IDSD01840
Strain NameB1KO-2
SpeciesT. thermophila
Genetic DescriptionBTU1/btu1[∆,::H4-neo]; MPR1-1/mpr1-1
MAC Knockins/outs
MIC Knockins/outsH4-neo replaces coding of BTU1, heterozygous
Gene of InterestBTU1 (TTHERM_00348510)
OriginBing Hai
PublicationsHai B and Gorovsky MA. 1997. Germ-line knockout heterokaryons of an essential alpha-tubulin gene enable high-frequency gene replacement and a test of gene transfer from somatic to germ-line nuclei in tetrahymena thermophila. Proc Natl Acad Sci U S A 94(4):1310-5.

Xia L, Hai B, Gao Y, Burnette D, Thazhath R, Duan J, Bre MH, Levilliers N, Gorovsky MA, Gaertig J. 2000. Polyglycylation of tubulin is essential and affects cell motility and division in tetrahymena thermophila. J Cell Biol 149(5):1097-6.
CommentsGermline transformant (heterozygote). Transformed with BHAB-1 (BTU1 knockout construct)

Mates with B1KO-1

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD01840
Requires MTA?No
Academic cost $45