Tetrahymena Stock Center| Stock ID | SD01838 |
| Strain Name | ATU-6D |
| AKA | |
| Species | T. thermophila |
| Genetic Description | atu1-1[Δ::neo1]/atu1-1[Δ::neo1]; mpr1-1/mpr1-1 or MPR1/MPR1 (atu1-1[Δ::neo1]/atu1[ E438D, E441D, E443D, E445D,E446D,E447D ]; ATU1 with E438D, E441D, E443D, E445D,E446D,E447D or neo replacing coding of some ATU1 genes, pm-r, cy-s,mp-? Slow growth ) |
| MAC Knockins/outs | ATU1 with E438D, E441D, E443D, E445D,E446D,E447D or neo replacing coding of some ATU1 genes |
| MIC Knockins/outs | neo replaces ATU1 coding |
| Gene of Interest | ATU1 (TTHERM_00558620) |
| Origin | Jianming Duan |
| Publications | Hai B and Gorovsky MA. 1997. Germ-line knockout heterokaryons of an essential alpha-tubulin gene enable high-frequency gene replacement and a test of gene transfer from somatic to germ-line nuclei in tetrahymena thermophila. Proc Natl Acad Sci U S A 94(4):1310-5. Xia L, Hai B, Gao Y, Burnette D, Thazhath R, Duan J, Bre MH, Levilliers N, Gorovsky MA, Gaertig J. 2000. Polyglycylation of tubulin is essential and affects cell motility and division in tetrahymena thermophila. J Cell Biol 149(5):1097-6. |
| Comments | It is a progeny of conjugants of AAK02.7XAAKO5.5 transformed with pATU-6D derived from pTUB100E3-PvuII (linearized)where the ATU1 gene is marked with Hae III and Pvu II sites and E438D, E441D, E443D, E445D,E446D,E447D From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed. |
| Research Resource Identifier | TSC_SD01838 |
| Requires MTA? | No |
| Academic cost | $45 |
