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We are now shipping live cells, plasmids, genomic DNA, and media packets, although shipping may take somewhat longer than normal. Contact us with any questions.


Stock IDSD01830
Strain NameA445-1
AKA
SpeciesT. thermophila
Genetic Descriptionatu1-1[Δ::neo1]/atu1-1[Δ::neo1]; mpr1-1/mpr1-1 or MPR1/MPR1 (atu1-1[Δ::neo1]/atu1[E445A]; mp-?)
MAC Knockins/outsATU1 with E445A or neo replacing coding of some ATU1 genes
MIC Knockins/outsneo replaces ATU1 coding
Gene of InterestATU1 (TTHERM_00558620)
OriginBing Hai
PublicationsHai B and Gorovsky MA. 1997. Germ-line knockout heterokaryons of an essential alpha-tubulin gene enable high-frequency gene replacement and a test of gene transfer from somatic to germ-line nuclei in tetrahymena thermophila. Proc Natl Acad Sci U S A 94(4):1310-5.

Xia L, Hai B, Gao Y, Burnette D, Thazhath R, Duan J, Bre MH, Levilliers N, Gorovsky MA, Gaertig J. 2000. Polyglycylation of tubulin is essential and affects cell motility and division in tetrahymena thermophila. J Cell Biol 149(5):1097-6.
CommentsIt is a progeny of conjugants of AAK02.7 X AAKO5.5 transformed with pA445 derived from pTUB100E3-PvuII (linearized) where the ATU1 gene is marked with Hae III and Pvu II sites and E445A.

The strain was supposed to be pm-r, but the thawed line is pm-s.

From the Gorovsky lab, University of Rochester.
Research Resource IdentifierTSC_SD01830
Requires MTA?No
Academic cost $45