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Stock IDSD01826
Strain Namep115 V5 CU428-2
SpeciesT. thermophila
Genetic Descriptionmpr1-1/mpr1-1 (giw1[3’neo3,V5c]; p115:: p115-V5-neo3, pm-r V5 tagged GIW1p, VII)
MAC Knockins/outsAll GIW1 genes replaced by c-terminal V5 tagged version with neo3 in 3’ flanking region
MIC Knockins/outs
Gene of Interestp115, GIWI (TTHERM_01276320 )
OriginKaz Mochizuki ( KM65)
PublicationsNoto T, Kurth HM, Kataoka K, Aronica L, DeSouza LV, Siu KW, Pearlman RE, Gorovsky MA, Mochizuki K. 2010. The tetrahymena argonaute-binding protein Giw1p directs a mature argonaute-siRNA complex to the nucleus. Cell 140(5):692-703.
CommentsAll p115 copy in Mac was replaced with p115-V5-neo3 construct in CU428.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD01826
Requires MTA?No
Academic cost $35