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We are now shipping live cells, plasmids, genomic DNA, and media packets, although shipping may take somewhat longer than normal. Contact us with any questions.

Stock IDSD01815
Strain Namec∆SAI 2-9-1-Mtt-DCL1-2
SpeciesT. thermophila
Genetic Descriptiondcl1[∆?::neo3]/dcl1[∆?::neo3] (dcl1[∆?]; MTT1[∆::DCL1]; ∆DCL1, pm-s, 6mp?, MTT1 with wt DCL1, ?)
MAC Knockins/outshomozygous ∆DCL1 ,neo 3 was deleted in mac and assorted to pm-s; MTT1-DCL1 replaced some of MTT1
MIC Knockins/outshomozygous neo3 KO of DCL1,
Gene of InterestDCL1 (TTHERM_00284230)
OriginKaz Mochizuki (KM68)
PublicationsMochizuki K and Gorovsky MA. 2005. A dicer-like protein in tetrahymena has distinct functions in genome rearrangement, chromosome segregation, and meiotic prophase. Genes Dev 19(1):77-89.
Commentshomozygous homokaryon of ∆DCL1 (c∆SAI2 9-1) ,neo 3 was deleted in mac and assorted to pm-s; with MTTi locus replaced with MTT1-DCL1 construct

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD01815
Requires MTA?No
Academic cost $45