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Stock IDSD01813
Strain NameBMCG-hy3-63
AKA
SpeciesT. thermophila
Genetic Description∆hht1 & ∆hht2 ∆hhf1 ∆hhf2 hht1[∆,neo2]/hht1[∆,neo2]; hhf1[∆,neo]/hhf1[∆,neo]; hhf2,hht2[∆, neo2]/ hhf2,hht2[∆, neo2] (hht1[∆,neo2]/hhf1[∆,neo]/hhf2,hht2[∆, neo2]/ chimeric H3[H3(1-75) H3.3(76-90) H3(91-135)] MTT1/mtt1-?[5’ neo2,∆,(::CNA1), GFPc]; pm-r, bsr-1, hybrid H3 replaces major H3. In MTT1 locus,GFP tagged CNA1)
MAC Knockins/outshybrid H3 replaces major H3.
In MTT1 locus,GFP tagged CNA1
MIC Knockins/outs∆hht1 ; ∆hht2 ∆hhf1; ∆hhf2
Gene of InterestCNA1 (TTHERM_00146340), HHT2 (TTHERM_00189180), HHT3 (TTHERM_00016170)
OriginBowen Cui
PublicationsLiu Y, Song X, Gorovsky MA, Karrer KM. 2005. Elimination of foreign DNA during somatic differentiation in tetrahymena thermophila shows position effect and is dosage dependent. Eukaryot Cell 4(2):421-31.
Commentsmajor h3, h4 germline KO cells were rescued with hy3. Hybrid H3 contruct with H3(1-75) H3.3 (76-90) H3 (91-135), then transformed with Bsr-mtt1-cna1-gfp construct.

CNA1-GFP seq. was used to replace MTT1 coding seq. Bsr1 cas.

Thaw cells without drugs, then grow them in 60-100ug/ml blasticidin, Use 0.5-1 ug/ml cadmium chloride to express and see the green centromere.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD01813
Requires MTA?No
Academic cost $35